Journal: The Journal of Neuroscience

Article Title: Microglia–Müller Glia Cell Interactions Control Neurotrophic Factor Production during Light-Induced Retinal Degeneration

doi: 10.1523/jneurosci.22-21-09228.2002

Figure Lengend Snippet: Figure 1. Activation of microglia during light-induced retinal degeneration. A–F, Immunohistochemical analysis of normal (A–C) and light-reared (D–F) P35 rat retina using the antibodies iba1 (red in A, C, D, F) and ED1 ( green in B, C, E, F). In light-degenerated retina, iba1 immunoreactivity was observed in the outer retina and double-labeled with ED1 ( yellow) (F, arrowheads). G, Quantitative analysis of the ED1-positive cultured microglial cells from normal (red bar) and light-reared ( green bar) P35 rat retina. Each data point represents the mean SEM of the values obtained from six independent experiments; *p 0.05. H, I, Double-label immunocytochemistry of cultured microglial cells using the antibodies iba1 (red) and ED1 ( green) from normal (H) and light-reared (I) P35 rat retina. GCL, Ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars, 30 m.

Article Snippet: They were then incubated overnight with a microgliaspecific rabbit polyclonal antibody, iba1 (1.0 g/ml) (Graeber et al., 1998; Ito et al., 1998, 2001; Nakajima et al., 1998) and a mouse monoclonal antibody against ED1 (Serotec; 100 ) and visualized with Cy3conjugated goat anti-rabbit IgG (Amersham Biosciences) and FITCconjugated goat anti-mouse IgG (Jackson ImmunoResearch).

Techniques: Activation Assay, Immunohistochemical staining, Labeling, Cell Culture, Double-label Immunocytochemistry

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: Immunhistochemical detection of myeloperoxidase (MPO), neutrophil elastase (NE) and ED1 cells in rat liver 6 h after one single oral dose of CCl 4 -adminstration induced rat liver injury. MPO + cells ( a ), ED1 + cells ( b ) and NE + cells ( c ) in control rat liver. MPO + cells ( d ), ED1 + cells ( e ) and NE + cells ( f ) at 6 h after treatment. The lower panel shows MPO + cells ( g ), ED1 + cells ( h ) and NE + cells ( i ) at 24 h after treatment. Original magnification, ×100. G original magnification, ×200 173 × 116 mm (300 × 300 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Control

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: MPO + and ED1 + cells in rat liver after γ -Irradiation induced rat liver injury. ED1 + cells ( a ) and MPO + cells ( b ) in control rat liver. ED1 + cells ( c ) and MPO + cells ( d ) at 3 h after γ -Irradiation. ED1 + cells ( e ) and MPO + cells ( f ) at 24 h after γ -Irradiation. Original magnification, ×100 173 × 174 mm (300 × 300 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Irradiation, Control

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: Double staining of liver sections with monoclonal antibodies directed against MPO or NE ( red ) and monoclonal antibody against ED1 ( green ) followed by fluorescence immunodetection in sections of rat liver at different time points after CCl 4 -administration. a NE + or ED1 + cells in CCl4-induced liver injury at 0 h. b MPO + or ED1 + cells in CCl 4 -induced liver injury at 0 h. c NE + or ED1 + cells in CCl 4 -induced liver injury 24 h after administration. d MPO + or ED1 + cells in CCl 4 -induced liver injury 24 h after administration. Original magnification, ×100 173 × 130 mm (300 × 300 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Double Staining, Bioprocessing, Fluorescence, Immunodetection

Journal: Histochemistry and Cell Biology

Article Title: Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

doi: 10.1007/s00418-011-0787-1

Figure Lengend Snippet: Immunofluorescence staining of liver sections after γ -Irradiation at different time points with monoclonal antibodies directed against MPO ( red ) and monoclonal antibody against ED1 ( green ). MPO + cells ( a ) or ED1 + cells ( b ) in control liver. MPO + cells ( c ) and ED1 + cells ( d ) at 3 h after γ -Irradiation. MPO + cells ( e ) and ED1 + cells ( f ) at 6 h after γ -Irradiation. Original magnification, ×100 15 × 16 mm (600 × 600 DPI)

Article Snippet: Mouse monoclonal antibody ED1 against rat monocyte/macrophage was purchased from Serotec (Düsseldorf, Germany).

Techniques: Immunofluorescence, Staining, Irradiation, Bioprocessing, Control

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Macrophages contribute to the initiation of ischaemic acute renal failure in rats.

doi: 10.1093/ndt/gfk047

Figure Lengend Snippet: Fig. 1. Effect of LC on percentage peripheral blood monocytes and liver macrophages. Rats were injected with either LV or LC via tail vein. At 24 h later, blood samples and liver tissue were obtained to determine blood monocyte–macrophage depletion. (A) Percentage blood monocytes, (B) LV and (C) LC, liver macrophages (ED-1 staining, 100). (D) Number of ED-1-positive cells per HPF (200) in liver. *P<0.05 compared with LV, n ¼ 5 animals per group.

Article Snippet: Immunohistochemical detection of monocytes–macrophages was performed on paraformaldehyde (4%)-fixed and paraffinembedded kidney and liver tissue sections using the mouse monoclonal antibody against ectodysplasin-1 (ED-1) (Serotec, UK).

Techniques: Injection, Staining